David biweekly meeting
Reconstitution of endocytic actin network project
We've implemented the suggested changes except for the following:
Emily is fixing cartoon panel (work in progress, needs to add proteins)
- explanation of actin tail variability
- we weren't sure if the reviewer response should go into the main text or summarized since it seems overly detailed
- scWASP tethering explanation
- Emily came up with a rephrasing here
- We have data supporting that Ni-NTA/His-tag interaction is not necessary for
the assay. Is this worthwhile to make a supplementary figure?
Emily's Western blot showing scWASP binding to beads is independent of Ni-NTA lipid
- my representative light microscopy data (I have lots of examples)
+Ni-NTA lipid
-Ni-NTA lipid
Mechanical role of Type I myosin in CME theory project
recent progress
- re-installed and configured cytosim to run and analyze more simulations
- exploring comparisons between simulations and type 1 myosin measurements
- model seems to predict pretty closely the optimal unbinding rate and force sensitivity for endocytic myosin
next steps
- plot myosin step size and compare to measurements to make sure that's realistic
- subtract each mean internalization from mean internalization without
myosins to show assistive effect of myosins
- maybe set this up by scanning number of myosins instead
- scan a bigger parameter range; when do they become harmful to CME?
- also scan different tensions in order to see which regime provides robustness
against resistive load
- can we predict which behavior is more assistive to mammalian-like (low load) vs. yeast-like (high load) CME?
- generate a force vs. attachment lifetime plot for each of the simulations in
order to compare to Ross's and other myo data
- I only made this for the simulated motility assay so far, but I think it should still behave the same in the CME model
- characterize actin organization somehow?